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Sequencing Grade Recombinant Carboxypeptidase B EC 3.4.17.2

Categories Recombinant Carboxypeptidase B
Brand Name: YaxinBio
Model Number: SRCPB01
Certification: ISO 9001
Place of Origin: China
MOQ: 100ug
Payment Terms: T/T
Packaging Details: ice packaging
Price: 198$/100ug
Supply Ability: 1000g per week
Product: Sequencing Grade Carboxypeptidase B
Manufacturer: YaxinBio
Cat. No.: SRCPB01
Specific activity: NLT 200 units/mg pro
Contaminant activity: No trypsin, chymotrypsin, carboxypeptidase A, or other proteases contaminant.
RELATED PRODUCT: V8 (sequencing grade)
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Sequencing Grade Recombinant Carboxypeptidase B EC 3.4.17.2

Sequencing Grade Recombinant Carboxypeptidase B EC 3.4.17.2


Description


Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The molecular weight is 33.8kD, the pH optimum is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.


Application


Protein structure and sequence analysis, such as hydrolyze basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides.

Antibody quality control.


Recommend Usage


Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7-9.


Storage Instructions


Recommend recombinant carboxypeptidase B lyophilized should be stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and no activity lose after 10 times repeated freezing and thawing.



Email:Zhaozhi@yaxinbio.com

Product Tags:

recombinant insulin

  

carboxypeptidase b

  
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